MANUFACTURING OOCYTES THROUGH SOMATIC CELL NUCLEAR TRANSFER
نویسندگان
چکیده
منابع مشابه
I-12: Nuclear Reprogramming in Bovin Somatic Cell Nuclear Transfer
Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
متن کاملSuccessful cloning of coyotes through interspecies somatic cell nuclear transfer using domestic dog oocytes.
Interspecies somatic cell nuclear transfer (iSCNT) is an emerging assisted reproductive technology (ART) for preserving Nature's diversity. The scarcity of oocytes from some species makes utilisation of readily available oocytes inevitable. In the present study, we describe the successful cloning of coyotes (Canis latrans) through iSCNT using oocytes from domestic dogs (Canis lupus familiaris o...
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Background: The mammalian oocyte has the unique feature of supporting fertilization and normal development while being able of reprogramming the nuclei of somatic cells towards pluripotency, and occasionally even totipotency. Whilst oocyte quality is known to decay with somatic ageing, it is not a given that different biological functions decay concurrently. In this study, we tested whether ooc...
متن کاملSomatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits
The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients for somatic cell nuclear transfer (SCNT). Within a common SCNT protocol, a comparison between oocytes recovered by direct aspiration (aspirated) from available ovarian follicles and oocytes flushed...
متن کاملHuman somatic cell nuclear transfer (cloning).
Within 2 years of the announced birth in 1997 of Dolly, the lamb cloned from the mam-mary cells of an adult ewe, research groups announced that they had cloned mice and calves by using differentiated somatic cells (1– 3). In the cloning technique used to produce Dolly, the nucleus of a somatic cell of the ewe was transferred to a sheep oocyte from which the nucleus had been removed, and the cel...
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ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2020
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2020.08.224